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. 2003 Jul 21;198(2):281–291. doi: 10.1084/jem.20030645

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — Frequency of DC subsets and their expression of cell surface markers from LN after immunization. (A) CD11c⁺ DCs from draining LNs of naive (no injection) and or mice immunized with PBS, CpG ODN, or LACK protein ± CpG ODN mice were enriched as described in the methods and stained with antibodies against CD11c, CD8 and B220. Cells were gated on CD11c⁺ cells and the percentage of CD11c⁺CD8⁺B220⁻, CD11c⁺CD8⁻B220⁻, and CD11c⁺B220⁺ is shown. (B) Expression of cell surface markers was analyzed on subsets of CD11c⁺ DCs based on expression of CD8 and B220. Light blue, no injection; black, PBS; blue, CpG ODN; green, LACK; red, LACK + CpG ODN. Data are representative of three independent experiments.