Figure 5.

Production of IL-12, IFN-γ, and IFN-α from CD11c⁺ DC subsets after immunization. Sorted CD11c⁺CD8⁺B220⁻, CD11c⁺CD8⁻B220⁻, and CD11c⁺B220⁺ (5 × 10³) cells were isolated from draining LN 2 d after immunization with PBS, CPG ODN, or LACK ± CpG ODN and cocultured with nothing, anti-CD40 (1 μg/ml), CpG ODN (5 μg/ml), or both. (A) IL-12 p40 was measured from all immunized groups. IL-12 p70 (B) and IFN-γ (B) were only detected from mice immunized with LACK + CpG ODN. (C) IFN-α was measured by ELISA from CD11c⁺B220⁺ cells in response to stimulation with CpG ODN 1585 and Poly I:C. Results are expressed as geometric mean ± SEM and are representative of three independent experiments.