Figure 7.

Frequency of LACK-specific CD4⁺ and CD8⁺ IFN-γ–producing cells from BALB/c mice after transfer of CD11c⁺ DCs and infection. Pooled spleen cells from BALB/c mice (n = 3) that received two immunizations with sorted CD11c⁺ DCs from BALB/c mice immunized with LACK ± CpG ODN were harvested 3 wk after infection with L. major. Spleen cells were cultured (6 × 10⁶) with bone marrow–derived DCs from BALB/c mice (1.2 × 10⁶) with or without exposure to LACK protein, anti-CD40, and CpG ODN stimulation. Cells were processed and stained as described to enumerate the frequency of LACK-specific CD4⁺ and CD8⁺ T cell IFN-γ responses. Numbers represent the percentage of CD4⁺ or CD8⁺ T cells positive for IFN-γ. * indicates P < 0.05 for cells of mice that received CD11c⁺ cells from LACK + CpG ODN-immunized mice compared with all other groups.