Figure 7.

Effects of TNF-α neutralization on the ability of different T cell subsets to control LVS growth in (A) wild-type BMMØ and (B) IFNγR KO BMMØs. Infected BMMØs were cocultured with splenocytes obtained from either uninfected mice (naive spleen), whole primed splenocytes from immune wild-type mice (primed spleen), or various T cell subsets enriched from immune wild-type mice (CD4⁺ cells, CD8⁺ cells, and CD4⁻CD8⁻Thy1.2⁺ cells). All splenocyte populations were added at a 1:2 ratio (splenocyte to BMMØ). Anti–TNF-α Abs were added to the wells at the time of addition of the splenocytes to the infected BMMØs. 72 h after infection, the BMMØ were assessed for levels of intracellular bacteria as described in the legend to Fig. 1. Values shown are the mean numbers of CFU/ml ± the SEM of viable bacteria (triplicate samples). Asterisks (*) indicate P values <0.01 as compared with cocultures containing naive spleen cells. These results are representative of three experiments of similar design.