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. 2003 Aug 4;198(3):443–453. doi: 10.1084/jem.20022018

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Translational suppression of Hck expression by the antisense oligonucleotide probe inhibits HIV-1_BaL replication in M-MΦ. (A) Immunoblot analysis of Hck in M-MΦ treated with oligonucleotide probes for Hck (2 μM) (AS, AS-Hck; NS, NS-Hck; S, S-Hck; and L, lipofectin alone) at 24 h (left) and in HIV-1_BaL–infected M-MΦ pretreated with the corresponding probes at 7 d PI, respectively (right). The data shown are representative one of three independent experiments. (B) Kinetics of the p24 levels in the culture supernatants of the oligonucleotide-pretreated HIV-1_BaL–infected M-MΦ. (C) Detection of viral DNA in the oligonucleotide-pretreated HIV-1_BaL–infected M-MΦ by nested PCR. The data shown are representative one of seven independent experiments. (D) p24 levels in AS-Hck or AS-C/EBPβ–treated PHA-activated CD4 T cells infected by HIV-1_NL4–3 (1 ng/ml) at 7 d PI. AS-Hck or AS-C/EBPβ has no effect on T-tropic HIV-1 replication in PHA-activated CD4 T cells.