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. 2003 Aug 4;198(3):455–467. doi: 10.1084/jem.20010897

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Immunocytochemical detection of neurotrophin receptor expression in cytospin preparations from purified peripheral blood eosinophils and BALF cells. In BALF cells, a strong immunoreactivity for the extracellular proportions of trkA, trkB, trkC, and the p75NTR was observed in eosinophils (arrows), whereas no immunoreactivity for any of the neurotrophin receptors was noted in eosinophils from peripheral blood (PB-Eos; trkA mAb from Cymbus Biotechnology LTD, trkB pAb from Chemicon, trkC mAb from R&D Systems, and p75NTR mAb from NeoMarkers). None of the cytospin preparations incubated with a negative control antibody (IgG₁, IgG_2a, IgG_2b, or rabbit IgG, respectively) yielded positively stained cells (depicted are representative photographs from cells which were incubated with IgG₁).