TABLE 1.
Selected bacterial strains and plasmids used in this studya
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| CU1065 | W168 attSPβ trpC2 | Laboratory stock |
| ZB307A | W168 SPβ c2Δ2::Tn917::pSK10Δ6 | 49 |
| HB0509 | HB1000 perR::spc | 7 |
| HB2194 | CU1065 perR::spc | This study |
| HB2078 | CU1065 perR::kan | 18 |
| HB2060 | CU1065 perR::kan amyE::cat | This study |
| HB2501 | CU1065 fur::kan | 18 |
| HB2502 | CU1065 fur::kan amyE::cat | 25 |
| HB8132 | CU1065 zur::spc | 22 |
| HB2168 | CU1065 fur::kan perR::spc | This study |
| HB2169 | CU1065 fur::kan zur::spc | This study |
| HB2170 | CU1065 perR::kan zur::spc | This study |
| HB1122 | ZB307A SPβ c2Δ2::Tn917::φ(mrgAPer box-cat-lacZ) | 11 |
| HB2144 | ZB307A SPβ c2Δ2::Tn917::φ(mrgAFur box-cat-lacZ) | This study |
| HB2145 | ZB307A SPβ c2Δ2::Tn917::φ(mrgAZur1 box-cat-lacZ) | This study |
| HB2146 | ZB307A SPβ c2Δ2::Tn917::φ(mrgAZur2 box-cat-lacZ) | This study |
| HB8206 | ZB307A SPβ c2Δ2::Tn917::φ(zosA-cat-lacZ) | 21 |
| HB0618 | ZB307A SPβ c2Δ2::Tn917::φ(feuAFur box-cat-lacZ) | This study |
| HB0619 | ZB307A SPβ c2Δ2::Tn917::φ(feuAPer box-cat-lacZ) | This study |
| HB0620 | ZB307A SPβ c2Δ2::Tn917::φ(feuAZur1 box-cat-lacZ) | This study |
| HB2218 | ZB307A SPβ c2Δ2::Tn917::φ(feuAZur2 box-cat-lacZ) | This study |
| HB8108 | CU1065 SPβ c2Δ2::Tn917::φ(zosA-cat-lacZ) | 21 |
| HB2118 | CU1065 perR::kan SPβ c2Δ2::Tn917::φ(zosA-cat-lacZ) | 18 |
| HB2111 | CU1065 fur::kan SPβ c2Δ2::Tn917::φ(zosA-cat-lacZ) | 18 |
| HB2139 | CU1065 perR::spc fur::kan SPβ c2Δ2::Tn917::φ(zosA-cat-lacZ) | This study |
| E. coli | ||
| DH5α | φ80lacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+)supE44 relA1 deoR Δ(lacZYA-argF)U169 | Laboratory stock |
| Plasmids | ||
| pJPM122 | cat-lacZ operon fusion vector for SPβ | 46 |
| pLC2260 | pJPM122 with mrgAPer box promoter | 11 |
| pMF36 | pJPM122 with mrgAFur box promoter | This study |
| pMF37 | pJPM122 with mrgAZur1 box promoter | This study |
| pMF38 | pJPM122 with mrgAZur2 box promoter | This study |
| pNB7 | pJPM122 with feuAFur box promoter | This study |
| pNB8 | pJPM122 with feuAPer box promoter | This study |
| pNB9 | pJPM122 with feuAZur1 box promoter | This study |
| pMF46 | pJPM122 with feuAZur2 box promoter | This study |
a
Additional strains containing reporter fusions are described in Materials and Methods.