Table I.
Hierarchy of Response of the Q1.1B6 T Cell Clone to Various Ligands
| Cytokine (pg/ml)
|
|||||||
|---|---|---|---|---|---|---|---|
| Ligand | Sequence | Relative MHC binding |
Proliferative response |
IFN-γ | TNF-α | IL-2 | IL-4 |
| L144 | HSLGKLLGHPDKF | 0.8 | ++++ | 9,040 | 420 | 1,350 | 390 |
| *Y144 | HSLGKYLGHPDKF | 0.6 | +++ | N.D. | N.D. | N.D. | N.D. |
| Q144 | HSLGKQLGHPDKF | 0.6 | ++ | 9,052 | <50 | <50 | 200 |
| W144 | HSLGKWLGHPDKF | 1.0 | + | 420 | <50 | <50 | <50 |
| Anti-TCR antibody | – | – | ++ | 8,350 | <50 | <50 | 60 |
Data summarized from Nicholson et al. 1998 (reference 10) from a representative experiment, except *Y144 compiled from data obtained from 1B6 T cell hybridoma and 1B6 transgenic T cells. The Q1.1B6 T cell clone was derived from an SJL mouse immunized with Q144. L144 is a superagonist ligand. Anti-TCR antibody activation is similar to activation with the cognate ligand Q144. N.D., not determined.