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. 2002 Nov 4;196(9):1163–1174. doi: 10.1084/jem.20021074

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Kinetics of V2, V6, V20, V12, and V19 rearrangements. (a) Rearrangements were analyzed by multiplex PCR on DNA extracted from thymi of 28-d-old, day 1 BALB/c mice, and FTOC from F16 after 6 d of culture. Specific V family primers are indicated on panels, these were used in conjunction with 9 J segments primers, as indicated on lanes. (b) Comparison of V2S2 versus V2S7 members at D28. PCR products were revealed with a pool of appropriate J-segments probes. Asterisks indicate nonspecific products, as determined by distance migration.