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. 2003 Nov;185(21):6295–6307. doi: 10.1128/JB.185.21.6295-6307.2003

FIG. 1.

FIG. 1.

Intracellular growth in human epithelial cells and PC-PLC activity. (A) Monolayers of HEp-2 cells were infected at an MOI of 50:1 with strains 10403S (•), DP-L2161 (10403S Δhly) (○), and DP-L2318 (10403S Δhly ΔplcB) (▵). Intracellular growth was determined as described in Materials and Methods. (B) Monolayers of HeLa cells were infected at an MOI of 67:1 with strains 10403S (•), DP-L2161 (10403S Δhly) (○), and DP-L2318 (10403S Δhly ΔplcB) (▵). (C) Monolayers of HeLa cells were infected at an MOI of 67:1 with PrfA* strains SLCC-5764 (•), DH-L377 (SLCC-5764 Δhly) (○), and DH-L419 (SLCC-5764 Δhly ΔplcB) (▵). The data points in growth curves represent the means ± the standard deviations of three coverslips from one of two experiments. (D) Overnight cultures of strains DP-L2161 (10403S Δhly) and DH-L377 (SLCC-5764 Δhly) were diluted 1:10 in BHI medium and grown for 5 h at 37°C. Proteins from culture supernatants were TCA precipitated and separated by SDS-PAGE. PC-PLC activities were determined by using an egg yolk overlay assay as described in Materials and Methods. Lane 1, the equivalent of 8 ml of DP-L2161 culture supernatant was loaded; lane 2, the equivalent of 0.32 ml of DH-L377 culture supernatant was loaded (1/25 the amount of lane 1).