Figure 1.

Effect of anti-α₆ integrins mAb GoH3 on leukocyte migration through IL-1β–stimulated mouse cremasteric venules, as observed by intravital microscopy. Animals were treated with intrascrotal saline (white bars) or IL-1β (30 ng/animal, black bars) 4 h before the surgical preparation. In additional groups of mice, animals were pretreated with intravenous mAbs GoH3 (anti-α₆ integrins), whole antibody or F(ab′)₂, or an isotype-matched control mAb, all at the dose of 3 mg/kg, 15 min before the intrascrotal injection of IL-1β. The panels show leukocyte firm adhesion and transmigration from the same experiments. The data represent mean ± SEM, from n = 5–8 mice/group. A significant difference from responses obtained from saline-injected animals is shown by asterisks, *P < 0.05. Additional statistical comparisons are indicated by lines.