Figure 3.

Effect of GoH3 on (A) leukocyte transmigration through IL-1β–stimulated cremasteric venules and (B) neutrophil infiltration into IL-1β–stimulated peritoneal cavity of WT and PECAM-1–deficient mice. Wild-type or PECAM-1–deficient mice were injected with intra-scrotal (A) or intraperitoneal (B) saline (white bars) or intrascrotal or intraperitoneal IL-1β (30 ng/mouse and 10 ng/cavity, respectively), 4 h before quantification. In the IL-1β–injected groups, the mice were pretreated with an isotype-matched control antibody or GoH3 (both at 3 mg/kg intravenously) 15 min before administration of IL-1β. The data represent mean ± SEM from n = 3–6 mice/group in A and n = 5–10 in B. Significant differences between control IL-1β elicited responses in WT and PECAM-1–deficient mice are indicated by +P < 0.05 and ++P < 0.01. Additional statistical comparisons are indicated by lines.