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. 2003 Nov 17;198(10):1539–1550. doi: 10.1084/jem.20011866

Figure 2.

Figure 2.

BCR-controlled adhesion is dependent on Lyn and Syk and requires activation of PI3K, but not of MEK or ERK. (A) WT or Lyn/Syk double-deficient DT40 cells (Lyn−/Syk−) were not stimulated (C) or stimulated with anti-IgM (a-IgM) and plated on a surface coated with VCAM-1. (B) Activation of PKB and ERK in WT or Lyn/Syk double-deficient DT40 cells (Lyn−/Syk−) after stimulation with anti-IgM for the indicated period of time (min). The immunoblots are probed with anti-phospho-PKB (P-PKB) and anti-phospho-MAPK (P-ERK) and reprobed with anti-ERK2 and anti-PKB, as indicated. (C) Activation of PKB and ERK in Namalwa cells (left) or DT40 cells (right) which were pretreated with 20 μM LY294002 (LY), 100 nM Wortmannin (WM), 50 μM PD98059 (PD), or left untreated (−) for 30 min, and stimulated with anti-IgM (a-IgM) or not (C) for 5 min, as indicated. The blots are probed as in B. (D) Namalwa cells (left) or DT40 cells (right) were pretreated with 50 μM LY294002 (LY), 100 nM Wortmannin (WM), 50 μM PD98059 (PD), or left untreated for 30 min, and not stimulated (C) or stimulated with anti-IgM (a-IgM) and plated on a surface coated with VCAM-1.