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. 2003 Nov 17;198(10):1563–1572. doi: 10.1084/jem.20031220

Figure 2.

Figure 2.

(A) L. pneumophila activates TLR5 through FliC. CHO cells were stably transfected with an NF-κB luciferase promoter and either a control vector (pEF6, shaded bars) or TLR5RNF (white bars). Cells were stimulated with S. typhimurium FliC at 10 ng/ml−1, S. minnesota LPS at 100 ng/ml−1, or heat-killed L. pneumophila (multiplicity of infection = 250:1). Strains of L. pneumophila are as follows: LpWT, wild-type Corby strain; LpFlaA−, Corby strain FliC mutant; Boven, Bovenkarspel; Phil, Philadelphia; Knox, Knoxville. RLU, relative luciferase unit. (B and C) L. pneumophila stimulates macrophages through MyD88, but not TLR4. Macrophages were derived from mouse bone marrow and stimulated with PBS (white bars), S. minnesota LPS at 10 ng ml−1 (shaded bars), or LpWT (multiplicity of infection = 100:1; black bar). After 16 h of stimulation, supernatants were assayed by ELISA for TNF-α (B) or IL-6 (C). WT, wild type. T4, TLR4−/−. M88, MyD88−/− mouse strains. (A–C) Assays performed in triplicate with standard deviations indicated. (A) Transfected cells were stimulated for 4 h before determining luminescence levels.