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. 2003 Nov 17;198(10):1563–1572. doi: 10.1084/jem.20031220

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — FliC stimulation of cytokine production. (A–E) Cells were stimulated with LPS (•), purified S. typhimurium FliC (○), heat-killed wild-type L. pneumophila (⋄), or FlaA− (FliC mutant) L. pneumophila (×). (A and B) A549 cells; (C and D) Calu-3 cells; and (E) whole blood. (F–G) PBMCs were harvested from individuals with homozygous wild-type TLR5 (392RR; n = 6) or stop codon TLR5 heterozygotes (392R*; n = 4). Cells were stimulated with FliC at 30 ng/ml⁻¹ (F) or LPS at 10 ng/ml⁻¹ (G). (A–G) Cells were stimulated for 18 h and supernatants were assayed for cytokine production by ELISA. Data are representative of experiments performed at least twice in triplicate with standard deviations indicated. (F and G) The mean level and standard deviation of IL-6 were derived from averaging the responses of different individual's cells stimulated in triplicate.