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. 2003 Nov 17;198(10):1573–1582. doi: 10.1084/jem.20030298

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Kinetics of TAT-Δp85 transduction into whole-blood leukocytes in mice measured by flow cytometry. Whole-blood leukocytes were isolated at 0, 2, 6, 12, and 24 h after i.p. injection of FITC-labeled TAT-Δp85 to determine time-course and specificity of transmembrane uptake of TAT-Δp85. Nonspecific cell-surface fluorescence was quenched by resuspension in pH 6.8 HBSS. (a) Conjugated TAT-Δp85 demonstrates maximal transcellular uptake at 2 h and is progressively diminished over 24 h. (b) FITC-conjugated control Δp85 protein, which lacks the HIV-TAT domain, demonstrates no change in fluorescence versus after resuspension in pH 6.8 HBSS.