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. 2003 Nov 17;198(10):1609–1619. doi: 10.1084/jem.20030357

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Accumulation of isotype-switched B cells in MZ of spleen in μMT/lpr mice. (A) Spleen cells from normal and μMT/lpr mice were stained for CD21, CD23, and κ. FACS^® analysis for expression of CD21 and CD23 was performed on gated κ¹ cells. (B) Absolute numbers of newly formed (CD21^lo/CD23⁻), follicular (CD21⁺/CD23⁺), and MZ (CD21⁺/CD23⁻). Results are mean ± SEM of three mice in each group. (C) FACS^® analysis for CD5 expression in gated CD19⁺/κ¹ spleen cells from the indicated mice.