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Fas receptor ligation eliminates Isotype-switched B cell precursors in vitro. BM cells from μMT and μMT/lpr mice were cultured for a total of 5 d in the presence of IL-7. During the last 48 h, cultured cells were treated with 25 μg/ml FasIg or 2.5 μg/ml anti-Fas antibodies. Cultured cells were stained for CD19, B220, and κ. FACS® analysis was gated to viable CD19+ cells. The plots shown are representative of three different experiments.
