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. 2003 Nov 17;198(10):1609–1619. doi: 10.1084/jem.20030357

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — Development of isotype-switched B cell precursors is T cell independent. Normal, μMT, μMT/lpr, and μMT/lpr/TCRβδ^−/− mice were analyzed for development of isotype-switched B cells and production of serum IgG. (A) Titers of serum IgG were measured by ELISA (left) and are expressed as milligram/milliliter. Frequency of IgG-producing cells in spleens of these mice was quantitated by ELISPOT. The results are mean ± SEM of at least five mice in each group. (B) Representative ELISPOT membrane. Frequencies of plasma cells are mean ± SEM of at least five mice in each group. (C) Detection of γH chain in serum samples by Western blotting. Normal serum sample dilution is 1:10 and μMT/lpr serum dilution is 1:20. Samples of μMT and μMT/lpr/TCRβδ^−/− are not diluted. (D) Expression of κ-expressing cells in μMT/lpr/TCRβδ^−/− BM cultures. Cells grown in BM cultures prepared from the indicated mice were stained for B220, CD19, and κ. Analysis for κ expression is performed on 5,000 gated B220⁺ cells.