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. 2003 Nov 17;198(10):1507–1515. doi: 10.1084/jem.20030800

Figure 3.

Figure 3.

RAGE is a counter-receptor for β2-integrins. (A) Adhesion of nontransfected K562 cells, LFA-1-transfected K562 cells, Mac-1-transfected K562 cells, or p150,95-transfected K562 to immobilized RAGE is shown in the absence (gray bars) or presence (black bars) of β2-integrin–stimulating antibody Kim185 (10 μg/ml). (B) Adhesion of nontransfected K562 cells, LFA-1–transfected K562 cells, Mac-1–transfected K562 cells, or p150,95-transfected K562 cells, which were preincubated with Kim185 (10 μg/ml), to nontransfected CHO cells (gray bars) or RAGE-transfected CHO cells (black bars) is shown. (C) Adhesion of Mac-1–transfected K562 cells, which were preincubated with Kim185 (10 μg/ml), to RAGE-transfected CHO cells is shown in the absence (-) or presence of mAb to Mac-1 (20 μg/ml) or soluble RAGE (10 μg/ml). Cell adhesion is represented either as absorbance at 590 nm or as percentage of total added cells. All data are mean ± SD (n = 3) of a typical experiment, similar results were observed in at least three separate experiments.