Table III.

Proliferation and Cytokine Production by HIV Peptide–specific CD4⁺ T Cells in Viremic Patients

Patient	Peptide	Sequence	Percent CFSElow CD4+	DBLFa	CD69+ IL-2+	CD69+ IFN-γ1	IL-2+/IFN-γ1	IL-2+	IFN-γ1
111	G054	DRVHPVHAGPIAPGQ	0.02	0.0008	<0.01	0.40	<0.01	<0.01	0.35
	G067	WIILGLNKIVRMYSP	0.1	0.0005	<0.01	0.12	<0.01	<0.01	0.15
112	G008	YKLKHIVWASRELER	0.3	0.001	<0.01	0.10	<0.01	<0.01	0.15
	G067	WIILGLNKIVRMYSP	0.1	0.0005	<0.01	0.10	<0.01	<0.01	0.17
113	G065	PVGEIYKRWIILGLN	0.5	0.002	<0.01	0.30	<0.01	<0.01	0.35
	G067	WIILGLNKIVRMYSP	0.3	0.001	<0.01	0.40	<0.01	<0.01	0.46
114	G053	AAEWDRVHPVHAGPI	0.5	0.002	<0.01	0.10	<0.01	<0.01	0.19
	G076	YKTLRAEQASQEVKN	0.4	0.002	<0.01	0.10	<0.01	<0.01	0.17
115	G067	WIILGLNKIVRMYSP	0.4	0.002	<0.01	0.20	<0.01	<0.01	0.21
	G061	STLQEQIGWMTNNPP	0.2	0.0008	<0.01	0.20	<0.01	<0.01	0.26
116	N048	LAFHHVARELHPEYF	0.35	0.001	<0.01	0.12	<0.01	<0.01	0.15
	G065	PVGEIYKRWIILGLN	0.3	0.001	<0.01	0.11	<0.01	<0.01	0.17
117	G004	LDRWEKIRLPRGGKK	0.5	0.002	<0.01	0.42	<0.01	<0.01	0.40
	N036	WCYKLVPVEPDKVEE	0.4	0.002	<0.01	0.31	<0.01	<0.01	0.30
118	N022	VDLSHFLKEKGGLEG	0.4	0.002	<0.01	0.20	<0.01	<0.01	0.23
	G057	PGQMREPRGSDIAGT	0.2	0.0008	<0.01	0.23	<0.01	<0.01	0.24
		Median	0.321	0.001	NA	0.21	NA	NA	0.24

Values of percent CFSE^low CD4⁺ are the medians of five independent experiments. The percentages of cytokine production were measured after 12 h after stimulation of patients' PBMCs with HIV-specific peptides. The data depicted are the medians of three separate experiments. Values <0.01 indicate undetectable frequencies. NA, not applicable.

^aDBLF, deduced baseline frequency derived from the 6-d CFSE-based proliferation assay.