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. 2003 Dec 15;198(12):1785–1796. doi: 10.1084/jem.20021562

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — CS does not induce nonspecific binding of tetramers to LMNCs. (A) Different groups of CBA/J mice were PCl skin sensitized or i.v. injected with 0.5 μg of α-GalCer, and 4 h later LMNCs were obtained and stained with CD1d-α-GalCer tetramers or PE-CD1d–unloaded tetramers to evaluate nonspecific binding. We analyzed pooled LMNCs from four mice per group. (B) Different groups of Jα18^−/− or CD1d^−/− mice and appropriate WT controls were PCl sensitized, and 2 h later LMNCs were obtained. The LMNCs were stained to analyze Vα14i NKT cells by flow cytometry. The numbers indicate the percentages of tetramer-positive liver TCRβ⁺ cells analyzing pooled LMNCs from four mice per group.

Figure 4. — CS does not induce nonspecific binding of tetramers to LMNCs. (A) Different groups of CBA/J mice were PCl skin sensitized or i.v. injected with 0.5 μg of α-GalCer, and 4 h later LMNCs were obtained and stained with CD1d-α-GalCer tetramers or PE-CD1d–unloaded tetramers to evaluate nonspecific binding. We analyzed pooled LMNCs from four mice per group. (B) Different groups of Jα18^−/− or CD1d^−/− mice and appropriate WT controls were PCl sensitized, and 2 h later LMNCs were obtained. The LMNCs were stained to analyze Vα14i NKT cells by flow cytometry. The numbers indicate the percentages of tetramer-positive liver TCRβ⁺ cells analyzing pooled LMNCs from four mice per group.