Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Dec 15;198(12):1785–1796. doi: 10.1084/jem.20021562

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — 4-d immune WT T cells fail to transfer CS to 1-d immune NKT cell–deficient mice. (A) CD1d^−/− mice (Group C) and CD1d^+/+ WT controls (Group B) were skin sensitized and 1 d later received 4-d PCl immune-isolated T cells from WT CD1d^+/+ mice and then were ear challenged 1 d later. The T cells were obtained by treating WT immune cells with anti-CD19 plus complement. Nonimmune CD1d^+/+ mice (Group A) similarly received T cell transfers and were ear challenged. Nonimmune CD1d^−/− (Group D) and CD1d^+/+ mice (Group E) were challenged but received no transfers. (B) Jα18^−/− mice (Group C) and BALB/c WT controls (Group B) were contact sensitized and 1 d later received 4-d PCl immune–isolated T cells from BALB/c mice and then were ear challenged 1-d later. The T cells (99% TCRβ⁺) were obtained by magnetic separation. Nonimmune BALB/c mice (Group A) similarly received T cell transfers and were ear challenged. Nonimmune Jα18^−/− (Group D) and BALB/c mice (Group E) were challenged but received no transfers. In A and B, the ear responses were measured at 2 and 24 h from a representative experiment with four mice per group.

Figure 6. — 4-d immune WT T cells fail to transfer CS to 1-d immune NKT cell–deficient mice. (A) CD1d^−/− mice (Group C) and CD1d^+/+ WT controls (Group B) were skin sensitized and 1 d later received 4-d PCl immune-isolated T cells from WT CD1d^+/+ mice and then were ear challenged 1 d later. The T cells were obtained by treating WT immune cells with anti-CD19 plus complement. Nonimmune CD1d^+/+ mice (Group A) similarly received T cell transfers and were ear challenged. Nonimmune CD1d^−/− (Group D) and CD1d^+/+ mice (Group E) were challenged but received no transfers. (B) Jα18^−/− mice (Group C) and BALB/c WT controls (Group B) were contact sensitized and 1 d later received 4-d PCl immune–isolated T cells from BALB/c mice and then were ear challenged 1-d later. The T cells (99% TCRβ⁺) were obtained by magnetic separation. Nonimmune BALB/c mice (Group A) similarly received T cell transfers and were ear challenged. Nonimmune Jα18^−/− (Group D) and BALB/c mice (Group E) were challenged but received no transfers. In A and B, the ear responses were measured at 2 and 24 h from a representative experiment with four mice per group.