Figure 3.

Reduced IFN-γ, but elevated inflammatory cytokine and chemokine expression by p35^−/− mice. Cytokine and chemokine mRNA levels in hind paws day 42 after primary CII/CFA immunization were determined by quantitative real-time PCR and expressed in units relative to ubiquitin mRNA levels. (A) mRNA expression levels for IFN-γ and the IFN-γ–inducible chemokines IP-10 and Mig. (B) mRNA expression of genes encoding proinflammatory factors. RNA from 3 to 5 individual paw samples for each genotype were pooled for analysis, except for IFN-γ and IL-17, where the data represent the mean ± SEM; n = 3. The abundance of mRNA encoding IFN-γ and IL-17 were very low and, hence, were confirmed using expression data from individual mice. (Naive) Paws from unimmunized mice. WT samples, both inflamed and naive, consist of pooled RNA from both C57BL/6 and B6 × 129 F2 mice.