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. 2003 Aug 18;198(4):591–602. doi: 10.1084/jem.20022042

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — FACS^® analysis of immune responses. Red cell–depleted splenocytes were stained as indicated and 10⁵ events were collected per sample on a FACSCalibur™. Antigen-specific B cell expansion was measured by gating on λ⁺/NIP⁺, live splenocytes. Representative data of day 12 after immunization with NP₂₂-CGG are shown here and summarized in Fig. 3 and Table I.