Figure 2.

The efficiency of DC migration to draining lymph node is increased by TNF, IL-1, and by DCs themselves. (A) PBS or 10⁶ CFSE-labeled DCs were injected into the footpads of syngeneic mice. 24 h later, the mice received a second injection of 10⁶ CMTMR-labeled DCs in the same footpad. Mice were killed 24 h after the second injection and the number of CMTMR⁺ DCs migrated to the draining lymph nodes was analyzed. (B) The experiment was performed as in A after the injection of PBS, unlabeled 10⁶ DCs, TNF, or IL-1α. Mean of three experiments each performed with groups of two mice per condition is shown. (C) Footpads pretreated with either PBS (•) or TNF (○) were injected with increasing doses of CMTMR-labeled DCs and the number of migrated DCs recovered in the draining lymph node was measured 2 d later. (D) Footpads of mice pretreated with PBS (•), TNF (□), or 10⁶ unlabeled DCs (○) were injected with 10⁶ CMTMR DCs and the number of migrated DCs was evaluated daily. Values are from pooled lymph nodes of two mice per time point. (E) CMFDA was applied to the skin of mice that had been pretreated with either PBS (•) or TNF (○). The number of fluorescent endogenous CD11c⁺ DCs recovered in the draining lymph node is shown.