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CCL21 is up-regulated on lymphatic endothelial cells after injection of mature DCs or TNF. Mice were injected intradermally with PBS, 105 syngeneic DCs, or 10 ng TNF. 8 h later, skin samples were collected and embedded in paraffin. Serial sections were stained with hematoxylin and eosin (A–C), or hybridized with antisense (D–F) or sense (inset) 35S-labeled CCL21 riboprobe or stained with a polyclonal antibody to mouse CCL21 (G–I). ×20.
