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. 2003 Aug 18;198(4):521–531. doi: 10.1084/jem.20030701

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — Identification and functionality of a point mutation in patient's IRAK-4. (A) Illustration of C877T substitution mutation (M #1), resulting in a truncated form of IRAK-4. Arrow indicates approximate location of truncation in IRAK-4 protein. (B) Vectors encoding the WT (N) or C877T mutation (M #1) forms of IRAK-4 were expressed in HEK293T cells (5 μg vector/transfection) and cell lysates subjected to Western blot analysis using anti-Flag Ab. (C) Overexpression of WT (N) or C877T (M #1) forms of IRAK-4 in HEK293T cells failed to induce NF-κB–induced reporter activity. Cells were transiently transfected with pELAM-Luc, pCMV-βGal, and the indicated amounts of expression vectors encoding either IRAK-1 or normal (N) and mutated (M) forms of IRAK-4 (total amount of plasmid DNA was kept constant at 1.5 μg per transfection). After recovery for 48 h, NF-κB reporter activity was measured. The data represent the mean ± SEM of a representative experiment (n = 3).

Figure 7. — Identification and functionality of a point mutation in patient's IRAK-4. (A) Illustration of C877T substitution mutation (M #1), resulting in a truncated form of IRAK-4. Arrow indicates approximate location of truncation in IRAK-4 protein. (B) Vectors encoding the WT (N) or C877T mutation (M #1) forms of IRAK-4 were expressed in HEK293T cells (5 μg vector/transfection) and cell lysates subjected to Western blot analysis using anti-Flag Ab. (C) Overexpression of WT (N) or C877T (M #1) forms of IRAK-4 in HEK293T cells failed to induce NF-κB–induced reporter activity. Cells were transiently transfected with pELAM-Luc, pCMV-βGal, and the indicated amounts of expression vectors encoding either IRAK-1 or normal (N) and mutated (M) forms of IRAK-4 (total amount of plasmid DNA was kept constant at 1.5 μg per transfection). After recovery for 48 h, NF-κB reporter activity was measured. The data represent the mean ± SEM of a representative experiment (n = 3).