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. 2003 Aug 18;198(4):521–531. doi: 10.1084/jem.20030701

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Identification and functionality of deletion mutation in patient's IRAK-4. (A) Illustration of effect of AC deletion at nucleotides 620-621 in the patient (620-621del), resulting in a truncated form of IRAK-4 (M #2). Arrow indicates approximate location of truncation in IRAK-4 protein. (B) Vectors encoding the WT (N) or mutated (M #2) forms of IRAK-4 were expressed in HEK293T cells (10 μg vector/transfection), and cell lysates subjected to Western blot analysis using anti-Flag Ab as described in Fig. 8. (C) Overexpression of WT (N), but not the 620-621 deleted mutant (M #2), IRAK-4 expression vectors (10 μg/transfection) differentially modulate IRAK-1 kinase activity (see Fig. 8 B; n = 3).

Figure 9. — Identification and functionality of deletion mutation in patient's IRAK-4. (A) Illustration of effect of AC deletion at nucleotides 620-621 in the patient (620-621del), resulting in a truncated form of IRAK-4 (M #2). Arrow indicates approximate location of truncation in IRAK-4 protein. (B) Vectors encoding the WT (N) or mutated (M #2) forms of IRAK-4 were expressed in HEK293T cells (10 μg vector/transfection), and cell lysates subjected to Western blot analysis using anti-Flag Ab as described in Fig. 8. (C) Overexpression of WT (N), but not the 620-621 deleted mutant (M #2), IRAK-4 expression vectors (10 μg/transfection) differentially modulate IRAK-1 kinase activity (see Fig. 8 B; n = 3).