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. 2003 Aug 18;198(4):545–556. doi: 10.1084/jem.20030088

Figure 1.

Figure 1.

Survival and replication of Brucella strains within BMDM. BMDM were infected with either the wild-type 2308 or the virB10 mutant Brucella strains for various times. CFUs were enumerated after lysis (A) or samples were fixed and processed for immunofluorescence (B–D). (A) Representative growth curves of wild-type 2308 (○) and virB10 (□) strains inside BMDM. (B) Quantitation of 2308- or virB10-infected BMDM containing either no bacteria (open bars), less than five bacteria (light gray bars), between 5 and 10 bacteria (dark gray bars), or >10 bacteria (solid bars) at 2, 8, and 24 h after infection. Data are means ± SD of three independent experiments. (C) Quantitation of cathepsin D acquisition by BCVs. Data are means ± SD of three independent experiments. (D) Representative confocal images showing colocalization of Brucella with cathepsin D in BMDM infected with either the wild-type 2308 or the virB10 mutant Brucella strains for 24 h. Arrows indicate colocalization and arrowheads show cathepsin D− BCVs. Bar, 5 μm.