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. 2003 Aug 18;198(4):557–567. doi: 10.1084/jem.20030788

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Surface reactivity of PVR-Fc or Nectin-2-Fc molecules with DNAM-1 cell transfectants COS-7 cells transfected with DNAM-1 construct were stained with PVR-Fc, Nectin-2-Fc, or Nectin-4tr-Fc (negative control) molecules, followed by PE-conjugated goat anti–human second reagent, or with GN18 (anti-DNAM-1) mAb, followed by PE-conjugated goat anti–mouse isotype-specific second reagent. Samples were analyzed by flow cytometry. White profiles indicate cells incubated with the second reagent only.