Figure 3.

Red-green display summarizing the regulation of selected intraphagosomally induced genes. cDNA ratios were averaged, log2 transformed, and displayed according to the color code at the bottom of the display. Gray fields indicate missing data. Experimental conditions were as indicated at the top of the display. The two columns shown for intraphagosomal regulation 24 h after infection indicate data from amplicon arrays and oligonucleotide arrays, respectively. Activation-specific genes were selected as described (Figs. 1 C and 2 A). The low iron response genes are all the genes induced in IFN-γ–stimulated macrophages that were induced at least twofold during growth with low iron. The multiple stress response genes are the genes that most strongly reacted to all three of the following conditions: PBS, heat shock, and SDS. Genes labeled as heat shock, SDS, and starvation responses were genes induced more than twofold during heat shock, SDS treatment, or starvation, respectively, and induced in activated macrophages but induced less than twofold in response to all other in vitro stresses.