Skip to main content
. 2003 Sep 1;198(5):823–830. doi: 10.1084/jem.20020437

Figure 4.

Figure 4.

CXCR3 ligands prime pDCs to respond to low SDF-1/CXCL12 concentrations. (A) Experiments were performed as a typical transwell migration assay with 20 ng/ml SDF-1/CXCL12 and 1 μg/ml IP-10/CXCL10, except that the upper well can also contain a chemokine. When both the upper and lower wells contain the same chemokine, the migration was identical or lower to that in medium alone (not depicted). (B) Preincubation experiments wherein the cells were first incubated in the presence of 20 ng/ml SDF-1/CXCL12 and 1 μg/ml IP-10/CXCL10 for 1 h before performing the migration assay to both receptor ligands in transwell. When the same chemokine was used in the preincubation and in the migration assay, the migration was identical or lower to that in medium alone (not depicted). Results are from n ≥ 3. (C) pDCs were incubated in presence of 20 ng/ml SDF-1/CXCL12, 1 μg/ml IP-10/CXCL10, or medium for 1.5 h at 37°C before performing the staining for CXCR4 (representative of n = 3). (D) CLA expression was performed on the different DC populations and monocytes by triple or double staining (representative of n = 4).