Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Sep 1;198(5):783–796. doi: 10.1084/jem.20030891

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Role of CD39 in ATP-elicited changes of posthypoxic endothelial permeability. (A) HMEC-1 were loaded with CD39-specific siRNA, control ribonucleotide or mock treated (control) and exposed to hypoxia or normoxia (48 h). Monolayers were washed, surface protein was biotinylated, and cells were lysed. CD39 was immunoprecipitated and resolved by SDS-PAGE, and resultant Western blots were probed with avidin-peroxidase. As a control for specificity, CD73 protein induction by hypoxia was assessed in parallel. (B) Influence of CD39 suppression by siRNA on functional surface protein. HMEC-1 were loaded with CD39-specific siRNA, control-ribonucleotide, or mock treated and CD39 activity was determined by HPLC analysis of E-ATP conversion to E-AMP (in the presence of the CD73-inhibitor αβ-methylene-ADP). Data are derived from five to seven monolayers in each condition, and results are expressed as E-AMP: E-ATP ratio ± SD. (C) Influence of CD39 suppression by siRNA on endothelial barrier. HMEC-1 were loaded with CD39-specific siRNA, control ribonucleotide or mock treated (control), exposed to hypoxia or normoxia (48 h), and permeability to 70 kD FITC in the presence or absence of ATP (100 μM) was assessed (*, P < 0.01 compared with no ATP). Data are derived from six monolayers in each condition, and data are expressed as mean ± SD of percent control flux.