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. 2003 Sep 1;198(5):679–691. doi: 10.1084/jem.20030066

Figure 3.

Figure 3.

High resolution peptide mapping of B*4402 and B*4403 ligands reveals ∼5% bound peptides are unique or preferentially bound by one allotype. (A) After an initial RP-HPLC separation of HLA-B*4402 bound peptides from C1R transfectants, a second dimension RP-HPLC separation was performed (right) and mass spectra determined for the selected fractions (left). (B–G) MALDI-TOF mass spectra of ligands from selected fractions after a 2nd round of RP-HPLC (A, right). Peptides from HLA-B*4402 (positive polarity spectra) and HLA-B*4403 (negative polarity spectra).