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. 2003 Sep 15;198(6):963–969. doi: 10.1084/jem.20031244

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — DCs from MLN, but not from spleen, are necessary and sufficient to induce a CCR9⁺α₄β₇ ⁺CD62L^low phenotype on Ag-specific CD8⁺ T cells. CFSE-labeled OT-1 cells were stimulated with SIINFEKL peptide-pulsed CD11c⁺ DCs and CD11c⁻ non-DCs or anti-CD3/CD28 mAbs. After 4 d of culture, the fraction of responding OT-1 cells expressing CCR9, CD62L, α₄β₇, and CXCR3 was determined by flow cytometry. (A) Representative results obtained for CCR9 and CD62L. (B) Percentages ± SD of responding OT-1 cells expressing indicated phenotype after stimulation with DCs (DC, n = 6), DC-depleted APCs (−DC, n = 3), or anti-CD3/CD28 mAbs (n = 3–5). Gray and white bars represent APCs from MLN and spleen, respectively. (C) Percentages ± SD of OT-1 cells expressing indicated phenotype as a function of cell division (n = 3). (D) Responsiveness of MLN DC (gray bars) and spleen DC (white bars) stimulated OT-1 cells to CCL25 and CXCL10. One representative experiment is shown.