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. 2003 Sep 15;198(6):963–969. doi: 10.1084/jem.20031244

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — In vivo gut-homing OT-1 cells express a CCR9⁺α₄β₇ ⁺CD62L^low phenotype which is induced efficiently only in GALT and after adjuvant-triggered inflammation. OT-1 cells were injected i.v. into C57BL/6J-Ly5.1 mice, and 2 d later mice received OVA i.p., either alone or in combination with adjuvant. (A) Flow cytometry analysis of OT-1 cells in indicated organs 3 d after immunization with OVA and pI:C. (B) Phenotype of OT-1 cells in the MLN and IEL compartment 3 d after immunization with OVA alone or OVA with adjuvant. (C) CFSE-labeled OT-1 cells were transferred into C57BL/6J-Ly5.1 mice, and donor cells in MLN and spleen were analyzed by flow cytometry 2 d after immunization with OVA or OVA plus pI:C. Results are representative of two to three performed experiments.