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. 2003 Sep 15;198(6):869–875. doi: 10.1084/jem.20030085

Figure 3.

Figure 3.

Phenotypic analysis of P. berghei chimeric lines. (A) Western blot analysis of late stage parasite extracts using rabbit αPbM19 or αPfM19 antibodies (both diluted 1/4,000) detecting both full-length MSP-1 (∼200 kD) and MSP-119 (∼19 kD). (B) Localization of MSP-119 by IFA. Schizont stage parasites were incubated with a mixture of rabbit αPbM19 (1/1,000) and 4H9/19 (αPfM19; 1/100) antibodies, followed by a mixture of FITC-conjugated anti–rabbit and rhodamine-conjugated anti–mouse immunoglobulins (both diluted 1/200). (C) Course of blood parasitemia in mice after infection at day 0 with P. berghei wild-type, Pb-PbM19, or Pb-PfM19. Shown is the mean ± SD of the parasitemia observed in five mice.