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Effect of L. pneumophila infection on LPS-induced p42/44 MAP kinase activity of MH-S alveolar macrophages. The cells were infected with L. pneumophila (Lp) for 30 min, washed to remove nonphagocytosed bacteria, and stimulated with or without LPS. The cell lysates were collected and the p42/44 MAP kinase activity was measured, as described in text. Representative results of three experiments are shown.
