TABLE 1.
Effect of L. pneumophila infection on levels of IL-1α, IL-6, IL-10, and IL-12 p40/p70 production induced by LPS
| Treatment | Levela of:
|
|||
|---|---|---|---|---|
| IL-1α (ng/ml) | IL-6 (ng/ml) | IL-10 (pg/ml) | IL-12 (ng/ml) | |
| Control | 0.13 ± 0.09 | 0.29 ± 0.12 | 24.9 ± 14.5 | 0.22 ± 0.12 |
| L. pneumophila | 1.82 ± 0.34 | 3.76 ± 0.62 | 242.7 ± 19.6 | 0.62 ± 0.26 |
| LPS | 2.45 ± 0.51 | 22.1 ± 2.54 | 572.6 ± 79.6 | 5.75 ± 0.84* |
| LPS + L. pneumophila | 2.51 ± 0.59 | 23.5 ± 2.18 | 563.9 ± 68.1 | 3.18 ± 0.55* |
a
The amount of cytokine in the culture supernatants obtained at 24 h after infection was measured by ELISA. Results are expressed as means ± standard deviations for three independent experiments. *, P < 0.05.