Figure 5.

Immunization route influences the location of DC-induced antigen-specific T cell expansion. Mice were immunized with 1 μM 10⁵ Tyr₃₆₉Y-pulsed DCs, delivered either s.c. or i.v. 7 d later, spleen and LNs were evaluated for total Tyr₃₆₉Y-specific CD8⁺ T cells using either (A) HLA-A*0201–Tyr₃₆₉Y tetramers or (B) intracellular production of IFN-γ by CD8⁺ T cells after stimulation with peptide-pulsed targets. Numbers in dotplots represent the percentage of CD8⁺ tetramer⁺ events (A) or CD8⁺ IFN-γ⁺ events (B) in the total lymphocyte population of the indicated lymphoid compartment. (C) Total tetramer⁺ and IFN-γ⁺ CD8⁺ cells are shown in each compartment.