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. 2003 Oct 6;198(7):1119–1126. doi: 10.1084/jem.20030411

Figure 2.

Figure 2.

Bim is required for BCR stimulation–induced deletion of self-reactive B cells in the BM. Lethally irradiated control and mHEL transgenic mice, reconstituted with either bim −/− or bim +/+ Ig transgenic BM at least 8 wk previously, were analyzed for the presence of IgH transgene+ B cells. Representative FACS® profiles of BM (A) and spleen (B) from the indicated reconstituted mice, stained with antibodies specific for CD45R(B220) and IgDa, show the enhanced persistence of bim −/− IgDa+ B cells relative to controls (see Table I for quantification and statistics). Histograms show the level of IgM expression and HEL binding on the B220+ cells from the corresponding tissue. (C) Transgenic IgDa+ cells purified from the BM and spleens of mHEL transgenic mice reconstituted with bim −/−anti-HEL Ig transgenic stem cell recover HEL binding after overnight culture, whereas those from the spleens of bim +/+ anti-HEL Ig transgenic stem cell reconstituted mice do not. IgDa+ cells from BM of both types of donor stem cells recover HEL binding (see Table II for quantification and statistics).