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. 2003 Oct 6;198(7):1119–1126. doi: 10.1084/jem.20030411

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — BCR ligation promotes binding of Bim to Bcl-2. (A) Coimmunoprecipitation of Bim with Bcl-2. After lysis proteins were immunoprecipitated with Bcl-2 antibody, the immunoprecipitated proteins were fractionated by SDS-PAGE, blotted onto membranes, and probed with antibodies to Bim_L and Bim_EL (top) or Bcl-2 (bottom). (B) Western blot analysis of extracts from B cells that had been stimulated in culture with cross-linking F(ab′)₂ anti-IgM antibodies or left untreated, using antibodies to Bim and Bcl-2.