Table II.
Restoration of HEL Binding after Culture of Bim-deficient Splenic B Cells Sorted from mHEL + Mice
| Tissue
|
mHEL+ recipient reconstituted with: |
IgDa+ cells/organ
|
Restored HEL binding after culturea |
HEL reactive cellsb
|
|---|---|---|---|---|
| 106 | % | 106 | ||
| Bone marrow | Tg+ bim+/+ | 0.5 ± 0.2 | 86.5 ± 4.9 | 0.43 |
| Tg+ bim−/− | 1.1 ± 0.5 | 95.3 ± 3.1 | 1.05 | |
| Spleen | Tg+ bim+/+ | 0.3 ± 0.2 | 14.0 ± 2.8 | 0.04 |
| Tg+ bim−/− | 1.7 ± 0.9 | 58.7 ± 6.0 | 1.00 |
a
HEL binding is measured by flow cytometric staining after overnight culture, shown in Fig. 2 C.
b
Calculates number of cells retaining HEL specificity as revealed after cell culture.