Figure 2.

TSP secretion by activated DCs. (A) Monocyte-derived iDCs were cultured for 24 h in the absence or presence of LPS, SAC, or L-CD40L + IFN-γ. TSP production was determined in the culture supernatants by ELISA (sensitivity, 10 ng/ml). Data are from one representative experiment out of three. (B and C) Kinetics of cytokine production by SAC-activated DCs. Monocyte-derived iDCs were cultured for the indicated periods of time in the absence or presence of SAC. IL-12, TNF-α, IL-10, and TSP production was determined by ELISA. Data are from one representative experiment out of three.