Figure 4.

Impact of the Y500F mutation on Th cytokine production. (A) In vitro Th cell polarization. Naive WT and Y500F splenic T cells were differentiated into Th1 or Th2 cells and then stimulated with anti-TCRβ mAB for 48 h. Culture supernatants were then harvested and examined for IL-4, IL-5, IL-13, and IFN-γ production by ELISA. (B) Cytokine production in response to antigenic stimulation. Splenocytes and mesenteric LN cells of OVA-immunized WT and Y500F mice were cultured for 4 d in the presence of 50 μg/ml of OVA. Culture supernatants were assayed for IL-4, IL-5, IL-13, and IFN-γ by ELISA. In both panels, results are means ± SE (n = 4–5 mice/group) and are representative of three experiments. *P < 0.001; **P = 0.013; ***P = 0.002.