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. 2003 Nov 3;198(9):1349–1360. doi: 10.1084/jem.20030381

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — Serum IgG responses to H. pylori CagA and CagY antigens. (A) By immunoblot, the recombinant J99 CagY antigen was recognized by rabbit α-CagY serum (lane 1), but not by serum from the patient from whom strain J99 was isolated (lane 2). (B) Similarly, using whole cell lysates from strain J99, the rabbit α-CagY serum (lane 1) recognized multiple high molecular weight CagY bands, whereas patient J99 (lane 2) recognized multiple bands (including CagA, UreB, and UreA), but not CagY. (C) That α-CagY immune rabbit sera reacted with the CagY antigen whereas control rabbit sera did not, indicates the antigenicity of CagY in the ELISA format. To determine whether the CagY protein is recognized by immune responses in persons with persistent H. pylori colonization, sera were examined from 38 H. pylori ⁺ persons for IgG antibodies to CagY and to CagA (as a control). As expected, sera from 27 patients carrying cagA ⁺ strains reacted more strongly (P < 0.001) to a recombinant CagA antigen than did sera from 11 patients carrying cagA ⁻ strains. Comparison of IgG responses to the CagY antigen between persons carrying cagA ⁺ and cagA ⁻ strains showed heterogeneous, but generally low level, responses. Although several patients had limited recognition of the recombinant CagY protein, the two groups of patients were not significantly different (P = 0.11).

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