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. 2003 Nov 3;198(9):1313–1322. doi: 10.1084/jem.20031159

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — CD46 down-regulation by pilus mutants of strain N401. N401 (P⁺) and its mutants (Table I), MW4 (P⁻), GT104 (PilT_ind), GV2 (PilV⁻), or GU5 (PilU⁻), were analyzed for adherence (A) and CD46 down-regulation (B). Adherence was assessed by FACS^® at 4 h after infection (MOI = 100) whereas CD46 levels were analyzed by FACS^® after incubation of bacteria with ME-180 cells for 6 and 24 h (MOI = 100). Results at 6 h (not depicted) were similar to those at 24 h. Data are expressed as mean ± SD of triplicate samples from one of three independent experiments.