Table III.

Yield of Viable Cells, IgG⁺ Cells, and Secreted IgG1 (and IgE) in 4-d Cultures of LPS/IL4-stimulated B Cells^a

Exp	Genotype	Percent viability	Cells/ml × 10−6	Percent IgG+ cells	[IgG1]	[IgE]
					ng/ml	ng/ml
A	s/+	73	2.2	18.0	242	8
	tg s/+	76	2.2	10.1	85	<1
	tg s/s	58	2.1	9.2	97	<1
	tg s/s	52	2.5	10.0	108	<1
B	s/+	77	1.8	12.6	723	48
	tg s/+	68	1.7	10.2	587	20
	tg s/s	78	1.1	7.7	312	<1
	tg s/s	62	1.8	8.9	305	<1

^aSpleen cells from individual 3H9sdVk8sd scid mice (tg s/s) and sorted B220⁺ splenic cells from a 3H9sdVκ8 scid/+ (tg s/+) and nontransgenic scid/+ (s/+) control mouse were plated at 0.25 × 10⁶ cells/ml. The source of cells for each genotype shown was an individual mouse. (A and B represent additional, independent experiments to those described in Fig. 7 A). Cell counts were made as described in Table I. The percentage of IgG1⁺ cells and levels of secreted IgG1 and IgE were ascertained by flow cytometry and ELISA, respectively (Materials and Methods).