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. Author manuscript; available in PMC: 2008 Nov 30.
Published in final edited form as: Mol Cell. 2007 Nov 30;28(4):638–651. doi: 10.1016/j.molcel.2007.11.001

Figure 2.

Figure 2

Nuclease activities of MR and MRX complexes. (A) MRX was incubated with a 5′ [32P]-labeled 46 bp DNA substrate containing a 4 nt recessed 3′ end in 1 mM MnCl2 or 5 mM MgCl2 with wild-type Sae2 protein as indicated. (B) The Rad50S MR(R20M)X complex was assayed in comparison to the wild-type complex as in (A) in 1 mM MnCl2. (C) The nuclease-deficient complexes M(D16A)R and M(Mre11-3)R were assayed in comparison to the wild-type MR complex as in (A) in 1 mM MnCl2.